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KMID : 0880220130510050659
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Journal of Microbiology
2013 Volume.51 No. 5 p.659 ~ p.664
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Characterization of Streptococcus pneumoniae N-Acetylglucosamine-6-Phosphate Deacetylase as a Novel Diagnostic Marker
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Choi Chi-Won
An Hee-Young
Lee Yong-Ju
Lee Yeol-Gyun
Yun Sung-Ho
Park Edmond-Changkyun
Hong Yeon-hee
Kim Gun-Hwa
Park Jae-Eun
Baek Sun-Jong
Kim Hyun-Sik
Kim Seung-Il
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Abstract
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The identification of novel diagnostic markers of pathogenic bacteria is essential for improving the accuracy of diagnoses and for developing targeted vaccines. Streptococcus pneumoniae is a significant human pathogenic bacterium that causes pneumonia. N-acetylglucosamine-6-phosphate deacetylase (NagA) was identified in a protein mixture secreted by S. pneumoniae and its strong immunogenicity was confirmed in an immuno-proteomic assay against the anti-serum of the secreted protein mixture. In this study, recombinant S. pneumoniae NagA protein was expressed and purified to analyze its protein characteristics, immunospecificity, and immunogenicity, thereby facilitating its evaluation as a novel diagnostic marker for S. pneumoniae. Mass spectrometry analysis showed that S. pneumoniae NagA contains four internal disulfide bonds and that it does not undergo posttranslational modification. S. pneumoniae NagA antibodies successfully detected NagA from different S. pneumoniae strains, whereas NagA from other pathogenic bacteria species was not detected. In addition, mice infected with S. pneumoniae generated NagA antibodies in an effective manner. These results suggest that NagA has potential as a novel diagnostic marker for S. pneumoniae because of its high immunogenicity and immunospecificity.
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KEYWORD
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Streptococcus pneumoniae, secreted proteins, antiserum, diagnostic marker
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